You were actually right, Onii-chan means pretty much the same as Onii-san as in 1 - Maybe the same time 'imouto' became a popular trope in anime? 2
At the start of Seitokai Yakuindomo OVA episode 19, there is this At the end of Texhnolyze, Kano gives the following dialogue: I became free of all genes by having intercourse with Lukuss.
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